TRMT6, also known as tRNA methyltransferase 6 homolog, is an enzyme that reflects the role of post-transcriptional modification in transfer RNA (tRNA). TRMT6 belongs to the class I-like SAM (S-adenosylmethionine)-dependent methyltransferase superfamily and catalyses the methylation of specific nucleotides within tRNA molecules. This modification, particularly at the wobble position of adenosine residues, is crucial for accurate and efficient translation of mRNA into protein, helping stabilise codon-anticodon interactions and ensuring fidelity during protein synthesis.