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Lab Test

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The SIBO test is recommended if you suspect a bacterial overgrowth in the small intestine. Examples of symptoms include flatulence, bloating, abdominal pain or cramps, diarrhoea and/ or constipation. The SIBO breath test is easy to use and your sample will be analysed at a ISO-certified lab.

Our SIBO Test is often paired with the Gut Microbiome XL Test, providing a thorough analysis of your gut health.

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About the SIBO Test

The SIBO test is recommended if you suspect a bacterial overgrowth in the small intestine. SIBO (Small Intestinal Bacterial Overgrowth) occurs when there is an increase in the bacterial population in the small intestine. These bacteria produce various degradation products such as hydrogen, carbon dioxide and short-chain fatty acids, which give rise to various gastrointestinal problems.

There are different types of SIBO that you can test for, which is why it is important to know what kind of SIBO you have. Different types of SIBO require different types of treatment methods. It is common to have several different varieties, but one of them is more dominant. Our test analyses the presence of methane gas (where different bacteria and microbes create gases) and hydrogen gas (where the bacteria ferment carbohydrates and create different types of gases). These are usually seen as the most common types of SIBO.

Symptoms of SIBO

SIBO has been theorised to be a possible cause of Irritable Bowel Syndrome (IBS). People diagnosed with IBS can benefit from testing for SIBO as studies have shown that almost 80% of these people have an overgrowth of bacteria in the small intestine. Bacterial overgrowth in the small intestine can create  problems such as:

  • Flatulence
  • Abdominal swelling
  • Abdominal pain or cramps
  • Diarrhoea
  • Constipation

If SIBO is left untreated, it can eventually lead to more problems such as leaky gut, depression, headaches, food intolerances, acne, fatigue or autoimmune reactions.

SIBO – how to perform the SIBO Test

SIBO is a breath test and includes 15 ml of lactulose liquid. The test measures the levels of hydrogen and methane in the exhaled air on several occasions during the day after ingestion of lactulose. This will tell if there is a bacterial overgrowth in the small intestine. You carry out the test yourself at home and then send it to our lab for analysis. 

You get the results of the test sent to you digitally, in the form of a report. This report includes recommendations on how you can proceed to treat your imbalance, in case you have a bacterial overgrowth.

Important advice before taking the SIBO Test

In order to not receive a false test result, we advise you to carefully follow the instructions given below, about what (not) to do before taking the test. 


Wait at least four weeks if you have recently undergone antibiotic treatment, colonoscopy, barium studies, barium enemas or have had diarrhoea.


Avoid using laxatives, laxatives and / or laxatives as well as acid scavengers containing aluminium or magnesium hydroxide.


– Stop taking probiotics 48 hours before the test.
– Foods that you CAN eat before starting your 12 hour fast: Baked or fried chicken, fish or turkey (salt and pepper only), white bread (only), plain steamed white rice, eggs, clear chicken or beef broth without vegetable pieces. Vegetarians can eat tofu with salt and pepper.
– You can only drink plain water, coffee and tea (without sugar, artificial sweeteners, milk or cream).


– The last 12 hours are the fasting period. Ingest NOTHING except water for 12 hours before and during the test.
– Unless otherwise instructed by your doctor, do not take unnecessary medicines or supplements until the test is completed.
– Do not chew gum, do not eat sweets and do not use mouthwash until the test has been completed.


– NO smoking (including passive smoking) for at least one hour before or at any time during the breath test.
– NO sleep or strenuous exercise for at least one hour before or at any time during the breath test.
– Do not use toothpaste.

Who should test for SIBO?

People with IBS, people who have difficulty breaking down carbohydrates, those with gluten intolerance / celiac disease and people who experience any of the above-mentioned symptoms, are considered suitable to test for SIBO.

However, there are other intestinal imbalances such as fungal overgrowth that can cause similar problems such as SIBO. Other gut health tests that might be of interest could be the Candida test if you also want to analyse the possible presence of yeasts, or our largest intestinal test Gut Microbiome Test XL if you are interested in doing a thorough test of your gut.

How to test for SIBO?

This test is a sibo breath test, by which you must breath through a mouthpiece into a bag, filling it slowly. You will do so multiple times. For more details, check the instructions. 

How long does it take until I get my results?

Once we receive your sample, average response time is 10-11 business days to receive results.

What causes SIBO?

Several structural problems in the small intestine can hinder its ability to deal with waste and excess bacteria. SIBO can also be caused by surgery or disease, in which case the passage of food and waste is slowed down in the digestive tract. This results in a breeding ground for bacteria. An overuse of medicine can also disrupt the balance of gut flora. 

How to treat SIBO?

The most common way to treat SIBO is with antibiotics or herbs. The test report contains recommendations for how to treat SIBO.

Not Sure This Test is for You?

If you’re still uncertain whether this test is right for you, don’t hesitate to reach out to our friendly and knowledgeable customer support team. We’re here to guide you towards the tests that best match your health concerns and objectives. You can contact us through our contact page or use our live chat for immediate assistance.


How is the SIBO test carried out?

Our SIBO test is a home test kit. After ordering, we will send you a kit with everything you need to collect the samples (7 tubes). Then, simply return your sample to us in the pre-paid envelope.

Anything to consider before taking the test?

Yes, you need to follow a specific diet before taking the SIBO test to avoid a false positive result. Also, remember to avoid taking antibiotics and probiotics 2-4 weeks prior to the test. Carefully follow the instructions provided. Detailed instructions:
4 weeks before: No antibiotics
7 days before: No laxatives, No antacids (such as aluminum or magnesium hydroxides)
48 hrs before: Food: No dietary fiber (e.g.: whole grain products, legumes, cabbage), Only easily digestible foods (e.g.: rice, chicken breast), No sugar, no sweets, No probiotics, No alcohol
12 hrs before: Drinking: Only still water Food: Fast for 12 hours before the test. (Your stomach must be empty at the beginning of the test!): No chewing gum, No toothpaste and no mouthwash (rinse teeth with water only!), Only take the most important medications (consult with your Doctor!)
1 hrs before: Do not smoke (even passively), No physical effort, Do not sleep During the test: Please do not drink any water after taking the test solution! 1 hr after taking the drinking solution, it is allowed to drink still water again.

Should the lactulose solution be mixed with water?

No. The lactulose is meant to be taken as it is. However if you have trouble taking it as it is, it is ok to dilute it with some water, it will not affect the test result.

When should I take the test?

It should be taken on an empty stomach in the morning. You should do the first test tube (reference test) BEFORE drinking the lactulose solution.

Do you also offer SIBO test with glucose?


My SIBO plastic bag has holes in it, is something wrong?

No, it is correct.

How long before I receive my Results?

The delivery time is 10-15 working days from when the sample is has been received by the lab. A text message and email will be sent to the customer when it is received by lab.

Can I open the test tubes?

The test tubes contain a vacuum and must not be opened.

Example Report

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Example of SIBO Test


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  • Clara Rutherford
    Quick results, clear information and excellent customer service. Couldn’t ask for a better experience. :)
  • Jayden Mitchell
    A rollercoaster of digestive symptoms, from severe bloating to abdominal pain, left me feeling both frustrated and defeated. Several medical consultations offered temporary solutions but no concrete diagnosis. Through my own research, I stumbled upon the possibility of SIBO. The SIBO Test was a beacon of hope amidst the uncertainty. From the receipt of the test kit to sending in my samples, the entire process was user-friendly. Once the results were in, the pieces of the puzzle began to fit together. The test indicated a clear bacterial imbalance in my small intestine, a hallmark of SIBO. Armed with this clarity, my interactions with healthcare professionals were more fruitful, leading to targeted treatments. The relief I've experienced since then has been profound. I wholeheartedly believe that this test was the catalyst for my journey towards better digestive health. :)

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